CNS

Alzheimer’s Disease

Primary culture of cortical neurons or hippocampal neurons co-cultured with glial cells (astrocytes and microglia)
• Amyloid-β 1-42 peptide injuries (induced with oligomers of peptide, for information see Callizot et al., 2013)
- Acute and chronic injuries (microglia activation)
• Glutamate and NMDA injuries
• TNF-α injuries / microglia activation

Parkinson’s Disease

Primary culture of mesencephalic neurons
• 6-OHDA injuries
• MPP+ injuries
• Rotenone injuries

Primary culture of mesencephalic neurons with microglial cells
• α-synuclein injuries

Amyotrophic Lateral Sclerosis

Nerves/muscle co-culture. Human muscle fibers innervated by rat spinal cord (SC) explants
• Glutamate injuries
• Amyloid-β 1-42 peptide injuries

Primary culture of motorneurons wild type and transgenic animals (SOD 1)
• Glutamate injuries
• Amyloid-β 1-42 peptide injuries

CENTRAL DEMYELINATION
MULTIPLE SCLEROSIS

Co-culture of myelinating oligodendrocytes and central neurons
• Pharmacological demyelination (interferon-γ, LPS…)

Huntington’s Disease

Primary culture of mesencephalic neurons (spiny neurons, GAD positive neurons) co-cultured with glial cells (astrocytes and microglia)
• Glutamate injuries
• Growth factor deprivation

ADRENOLEUKODYSTROPHY

Co-culture of myelinating oligodendrocytes and central neurons
• Very Long Chain Fatty Acid injuries

Primary culture of central neurons
• Very Long Chain Fatty Acid injuries

Primary culture of cortical neurons co-cultured with glial cells (astrocytes and microglia)
• Very Long Chain Fatty Acid injuries

MULTIPLE SYSTEM ATROPHY

Co-culture of myelinating oligodendrocytes and central neurons
• Rotenone injuries