Mode of action

Neuronal physiology and neurodegeneration depend on the interaction of different cell types and the regulation of molecular pathways. At Neuro-Sys, we use pharmacological tools, modulation of gene expression, immunocytochemistry, gene and protein level analysis to carefully dissect the mode of action of your compounds.

We can investigate how a neuroactive molecule regulates various pathways such as : Neurotrophic signalling. Using specific and well-validated antagonists of receptors and inhibitors of enzymes that are highly relevant to neuronal survival, such as :

- NMDA receptors
- TrkB signalling,
- NOS/NO/ cGMP pathway
- PI3/Akt signalling
- PPARγ signalling
- ERK signalling
- UPR signalling

In addition, we have validated the use of siRNA for precisely invalidate the expression of genes relevant for your compounds and targets. Biomolecular analysis of gene and protein expression/phosphorylation will inform on the mode of action of your compounds.
› Neuroinflammation, via the activation of microglial cells and astrocytes, and the release of cytokines.
› Protein clearance via ER stress/UPR signalling and macro-autophagy.
› Cell death pathways (e.g. caspase-dependent apoptosis, necroptosis).
› Mitochondrial pathways and energy metabolism (production of reactive oxygen species, levels of ATP, NADPH).