In vitro models

Primary culture of cortical or hippocampal neurons

co-cultured with glial cells (astrocytes and microglia)

• Amyloid-β1-42 peptide injuries (induced with oligomers of peptide, for information see Callizot et al., 2013)

• Acute or chronic injuries (microglia activation)

• Glutamate and NMDA injuries

• TNF-α injuries / microglia activation

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In vivo models​

Intra-hippocampal injection of amyloid β1-42 oligomers in aged mice.

Combines the toxicity of amyloid beta oligomers and advanced ageing. Our model mimics the main pathologies of AD: neurodegeneration, activation of pro-inflammatory response, long-term and short-term memory deficit. Validated with donepezil, drug indicated for AD.

ALZHEIMER'S DISEASE

PARKINSON'S DISEASE

In vitro models

Primary culture of mesencephalic neurons (dopaminergic neurons) 

• Mitochondrial toxins (6-OHDA, MPP+, rotenone) (Callizot et al. 2019)

• Lysosomal toxin (CBE)

• α-synuclein oligomers / protofibrils  (neurotoxicity)

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Primary culture of mesencephalic neurons with microglial cells

• α-synuclein oligomers / protofibrils (neuroinflammation)

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In vivo models​

Currently under validation. Will be based on toxicity of

alpha-synuclein protofibrils.

AMYOTROPHIC LATERAL SCLEROSIS

In vitro models

Nerves/muscle co-culture. Human muscle fibers innervated by rat spinal cord (SC) explants

• Glutamate injury

• Amyloid-β1-42 peptide injuries

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Primary culture of motorneurons wild type and transgenic animals (SOD 1)

• Glutamate injury

• Amyloid-β1-42 peptide injuries

HUNTINGTON'S DISEASE

In vitro models

Primary culture of mesencephalic neurons (spiny neurons, GAD positive neurons) co-cultured with glial cells (astrocytes and microglia)

• Glutamate injury

• Growth factor deprivation

CENTRAL DEMYELINATION MULTIPLE SCLEROSIS

In vitro models

Co-culture of myelinating oligodendrocytes and central neurons​

• Induced demyelination by : 

      - Cuprizone

      - LPS

      - INF-γ

• Kinetic of myelin maturation

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ADRENOLEUKO DYSTROPHY

In vitro models

Primary culture of central neurons

• Very Long Chain Fatty Acid injuries

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Primary culture of cortical neurons co-cultured with glial cells

• Very Long Chain Fatty Acid injuries

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MULTIPLE SYSTEM ATROPHY

In vitro models

Co-culture of myelinating oligodendrocytes and central neurons

• Rotenone Injury

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