CNS

Alzheimer’s Disease

> Primary culture of cortical or hippocampal neurons co-cultured with glial cells (astrocytes and microglia)
• Amyloid-β1-42 peptide injuries (induced with oligomers of peptide, for information see Callizot et al., 2013)
- Acute or chronic injuries (microglia activation)
• Glutamate and NMDA injuries
• TNF-α injuries / microglia activation

Parkinson’s Disease

> Primary culture of mesencephalic neurons (dopaminergic neurons)
• Mitochondrial toxins (6-OHDA, MPP+, rotenone) (Callizot et al. 2019)
• Lysosomal toxin (CBE)
• α-synuclein oligomers (neurotoxicity)

> Primary culture of mesencephalic neurons with microglial cells
• α-synuclein oligomers (neuroinflammation)

Amyotrophic Lateral Sclerosis (Lou Gehrig’s disease)

> Nerves/muscle co-culture. Human muscle fibers innervated by rat spinal cord (SC) explants
• Glutamate injury
• Amyloid-β1-42 peptide injuries

> Primary culture of motorneurons wild type and transgenic animals (SOD 1)
• Glutamate injury
• Amyloid-β1-42 peptide injuries

CENTRAL DEMYELINATION
MULTIPLE SCLEROSIS

> Co-culture of myelinating oligodendrocytes and central neurons
• Cuprizone-induced demyelination
• Interferon-γ, LPS

Huntington’s Disease

> Primary culture of mesencephalic neurons (spiny neurons, GAD positive neurons) co-cultured with glial cells (astrocytes and microglia)
• Glutamate injury
• Growth factor deprivation

ADRENOLEUKODYSTROPHY

> Primary culture of central neurons
• Very Long Chain Fatty Acid injuries

> Primary culture of cortical neurons co-cultured with glial cells
• Very Long Chain Fatty Acid injuries

MULTIPLE SYSTEM ATROPHY

> Co-culture of myelinating oligodendrocytes and central neurons
• Rotenone injuries