IN VITRO models
Primary culture of cortical or hippocampal neurons co-cultured with glial cells (astrocytes and microglia):
Amyloid-β1-42 peptide injuries (induced with oligomers of peptide, for information see Callizot et al., 2013)
Acute or chronic injuries (microglia activation)
Glutamate and NMDA injuries
TNF-α injuries / microglia activation
IN VIVO models
Intra-hippocampal injection of amyloid β1-42 oligomers in aged mice.
Combines the toxicity of amyloid beta oligomers and advanced ageing. Our model mimics the main pathologies of AD: neurodegeneration, activation of pro-inflammatory response, long-term and short-term memory deficit.
Validated with Donepezil, drug indicated for AD.