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PRECLINICAL MODELS  |  CNS  |  PD

Parkinson's disease

IN VITRO models​

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Primary culture of mesencephalic neurons (dopaminergic neurons):

  • Mitochondrial toxins (6-OHDA, MPP+, rotenone) (Callizot et al., 2019)

  • Lysosomal toxin (CBE)

  • α-synuclein / protofibrils  (neurotoxicity)

  • Combined toxicity of α-synuclein protofibrils and chronic lysosomal impairment (CBE)

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Primary culture of mesencephalic neurons with microglial cells:

  • α-synuclein oligomers / protofibrils (neuroinflammation)​​

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IN VIVO models​

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Intra-nigral injection of alpha-synuclein fibrils and lysosomal impairment in aged mice.

Combines the toxicity of alpha-synuclein fibrils and chronic inhibition of GBA, a lysosomal protein, in aged mice.

Our model mimics the main pathologies of PD: loss of dopaminergic neurons in the substancia nigra, neuroinflammation, deficit in motor coordination and synucleinopathy.

 

Model validated with compounds in clinical trials.

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