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Neuronal physiology and neurodegeneration depend on the interaction of different cell types and the regulation of molecular pathways. At Neuro-Sys we use pharmacological tools, modulation of gene expression, immunocytochemistry, gene and protein level analysis to carefully dissect the mode of action of your compounds.


We investigate how a neuroactive molecule regulates various pathways such as neurotrophic signalling. We use specific and well-validated antagonists of receptors and inhibitors of enzymes that are highly relevant to neuronal survival, such as:

  • NMDA receptors

  • TrkB signalling

  • NOS/NO/ cGMP pathway

  • PI3/Akt signalling

  • PPARγ signalling

  • ERK signalling

  • UPR signalling

In addition, we use SIRNA to precisely modulate the expression of genes relevant for your compounds and targets. Biomolecular analysis of protein expression/phosphorylation will define the mode of action of your compounds:

  • Neuroinflammation, via the activation of microglial cells and astrocytes and the release of cytokines

  • Protein clearance via ER stress/UPR signalling and macro-autophagy

  • Cell death pathways (e.g. caspase-dependent apoptosis, necroptosis)

  • Mitochondrial pathways and energy metabolism (production of reactive oxygen species, levels of ATP, NADPH)