Mode of action

We can investigate how a neuroactive molecule regulates various pathways such as : Neurotrophic signalling. Using specific and well-validated antagonists of receptors and inhibitors of enzymes that are highly relevant to neuronal survival, such as :

In addition, we have validated the use of siRNA for precisely invalidate the expression of genes relevant for your compounds and targets. Biomolecular analysis of gene and protein expression/phosphorylation will inform on the mode of action of your compounds.
› Neuroinflammation, via the activation of microglial cells and astrocytes, and the release of cytokines.
› Protein clearance via ER stress/UPR signalling and macro-autophagy.
› Cell death pathways (e.g. caspase-dependent apoptosis, necroptosis).
› Mitochondrial pathways and energy metabolism (production of reactive oxygen species, levels of ATP, NADPH).